- 产品名称Anti-A RAF抗体
参阅全部 A RAF 一抗
兔多克隆抗体to A RAF
- 特异性ab59343 detects endogenous levels of total A RAF protein.
WB, IHC-P, ELISAmore details
Synthesized non-phosphopeptide derived from human A RAF around the phosphorylation site of tyrosine 301/tyrosine 302 (G-Y-YP-W-E).
- Human breast carcinoma tissue
Extracts from HeLa cells
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- 存储溶液Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
- 纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Detects a band of approximately 68 kDa.
||Use at an assay dependent concentration.
- 功能Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
- 组织特异性Predominantly in urogenital tissues.
- 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain.
Information by UniProt
- A raf 1 antibody
- A Raf proto oncogene serine/threonine protein kinase antibody
- ARAF 1 antibody
Anti-A RAF antibody 图像
Western blot - A RAF antibody (ab59343)
All lanes : Anti-A RAF antibody (ab59343) at 1/500 dilution
Lane 1 : Extracts from HeLa cells treated
with PMA (125ng/ml, 30mins)
Lane 2 : Extracts from HeLa cells treated
with PMA (125ng/ml, 30mins), plus immunising peptide
Observed band size : 68 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - A RAF antibody (ab59343)
ab59343, at a 1/50-1/100 dilution, staining human A RAF in breast carcinoma with (+) or without (-) immunising peptide), using Immunohistochemistry, Paraffin embedded tissue
Anti-A RAF antibody (ab59343)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"