Application
Western blot
Sample
Human Tissue lysate - whole (Breast tissue)
Loading amount
10 µg
Specification
Breast tissue
Gel Running Conditions
Non-reduced Denaturing (12 %)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/30000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C
Secondary antibody
Name
Non-Abcam antibody was used: Anti-mouse IgG (#C0209, Santa Cruz Biotech)
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
SuperSignal West Pico Chemiluminescent S
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: ~ 60 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 21 2009