Anti-ZZEF1抗体(ab122194)
Key features and details
- Rabbit polyclonal to ZZEF1
- Suitable for: ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ZZEF1抗体
参阅全部 ZZEF1 一抗 -
描述
兔多克隆抗体to ZZEF1 -
宿主
Rabbit -
经测试应用
适用于: ICC, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence, corresponding to amino acids 2493-2568 (DYFFLEVQKR FDGDELTTDE RIRSLAQRWQ PSKSLRLEEQ SAKAVDTDMI ILPCLSRPAR CDQATAESNP VTQKLI) of Human ZZEF1.
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阳性对照
- IHC-P: Human testis and small intestine tissues; ICC: Human A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122194于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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ICC
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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组织特异性
Expressed at low levels in cerebellum. -
序列相似性
Contains 1 DOC domain.
Contains 1 EF-hand domain.
Contains 2 ZZ-type zinc fingers. - Information by UniProt
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数据库链接
- Entrez Gene: 23140 Human
- SwissProt: O43149 Human
- Unigene: 277624 Human
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别名
- FLJ10821 antibody
- KIAA0399 antibody
- Zinc finger ZZ-type and EF-hand domain-containing protein 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling ZZEF1 with ab122194 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and mitochondria. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine tissue labelling ZZEF1 with ab122194 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling ZZEF1 with ab122194 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling ZZEF1 with ab122194 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab122194 尚未被引用在任何文献中。