Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 3.0% BSA in TBS
Secondary antibody
Name
Non-Abcam antibody was used: Cy3 Donkey anti-Rabbit (Jackson Immuno)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
The image consists of prometaphase, metaphase and interphase HeLa cells stained with ZW10 (Red), DNA (Blue) and Microtubules (Green). The Zw10 does not confidently localize to kinetochores in the image. Addition stains with CREST also showed very little colonization with kinetochore and ZW10 and what may appear to be kinetochore localization in prometaphase failed to localize to CREST positive kinetochores.
Abcam response
Thank you for your feedback. We are going to re-test this antibody in the Lab.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Dec 11 2008