Application
Western blot
Sample
Human Cell lysate - whole cell (colon, ovary)
Gel Running Conditions
Reduced Denaturing (12 % gel)
Loading amount
20 µg
Specification
colon, ovary
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% skim milk
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: anti-Rabbit HRP-conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 60 kDa Non-specific: 80, 55, 30 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 14 2012