Anti-ZAP70 (phospho Y492)抗体[EP2291Y] (ab76306)
Key features and details
- Rabbit monoclonal [EP2291Y] to ZAP70 (phospho Y492)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-ZAP70 (phospho Y492)抗体[EP2291Y]
参阅全部 ZAP70 一抗 -
描述
兔单克隆抗体[EP2291Y] to ZAP70 (phospho Y492) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Jurkat cell lysates, treated with pervanadate.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP2291Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76306于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/20000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
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说明 |
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WB
1/10000 - 1/20000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). |
靶标
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功能
Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not. -
组织特异性
Expressed in T- and natural killer cells. -
疾病相关
Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Contains 1 protein kinase domain.
Contains 2 SH2 domains. -
结构域
The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH. -
翻译后修饰
Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity. -
细胞定位
Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes. - Information by UniProt
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数据库链接
- Entrez Gene: 7535 Human
- Omim: 176947 Human
- SwissProt: P43403 Human
- Unigene: 234569 Human
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别名
- 70 kDa zeta associated protein antibody
- 70 kDa zeta-associated protein antibody
- EC 2.7.10.2 antibody
see all
图片
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All lanes : Anti-ZAP70 (phospho Y492) antibody [EP2291Y] (ab76306) at 1/20000 dilution
Lane 1 : Jurkat cell lysates, untreated
Lane 2 : Jurkat cell lysates, treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab76306 被引用在 1 文献中.
- Chiang YJ et al. CBAP functions as a novel component in chemokine-induced ZAP70-mediated T-Cell adhesion and migration. PLoS One 8:e61761 (2013). WB . PubMed: 23620790