重组HRP Anti-ZAP70抗体[YE291] (ab208713)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [YE291] to ZAP70
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
概述
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产品名称
HRP Anti-ZAP70抗体[YE291]
参阅全部 ZAP70 一抗 -
描述
HRP兔单克隆抗体[YE291] to ZAP70 -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human ZAP70 (C terminal). The exact sequence is proprietary.
Database link: P43403 -
阳性对照
- WB: Jurkat whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
YE291 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab208713于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
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说明 |
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WB
1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). |
靶标
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功能
Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not. -
组织特异性
Expressed in T- and natural killer cells. -
疾病相关
Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Contains 1 protein kinase domain.
Contains 2 SH2 domains. -
结构域
The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH. -
翻译后修饰
Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity. -
细胞定位
Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes. - Information by UniProt
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数据库链接
- Entrez Gene: 7535 Human
- Omim: 176947 Human
- SwissProt: P43403 Human
- Unigene: 234569 Human
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别名
- 70 kDa zeta associated protein antibody
- 70 kDa zeta-associated protein antibody
- EC 2.7.10.2 antibody
see all
图片
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HRP Anti-ZAP70 antibody [YE291] (ab208713) at 1/5000 dilution + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab208713 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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