Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat cell lysate)
Gel Running Conditions
Reduced Denaturing (10% Bis Tris)
Loading amount
80 µg
Specification
Jurkat cell lysate
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: DAKO anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 second(s)
Bands
Specific: 45 kDa
Positive control
this
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提交于 Aug 03 2015