Application
Western blot
Sample
Mouse Cell lysate - whole cell (murine erythroleukemia (MEL cell))
Loading amount
30 µg
Specification
murine erythroleukemia (MEL cell)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/2500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% skim milk in PBS-T
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 48 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Min Young Kim
Verified customer
提交于 Jan 02 2013