Application
Western blot
Sample
Human Cell lysate - nuclear (Melanoma)
Gel Running Conditions
Reduced Denaturing (15% gel)
Loading amount
3000 cells
Specification
Melanoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/2000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 65 kDa Non-specific: 41, 44 kDa
Positive control
501mel cells infected with virus overexpressing hemagluttinin (HA)-tagged YL-1 - labeled "HA-YL1".
Additional data
Additional Notes
Lane 1 - 501 mel (human derived melanoma cell line) infected with vector overexpressing hemagluttinin-tagged YL1 (HA-YL1). 65 kDa band is YL-1. 41 and 44 kDa bands are possibly aspecific. Lane 2 - 501 mel with short hairpin (sh) RNA for YL-1 (of 2 commercially available varieties). 65 kDa band is YL-1. Lane 3 - 501mel with shRNA of scrambled target sequence. 65 kDa band is YL-1.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 29 2016