Application
Western blot
Sample
Human Cell lysate - whole cell (Liver, kidney)
Loading amount
40 µg
Specification
Liver, kidney
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Other product details
Dilution
1/200
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: TBS-Tween
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Dilution
1/10000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 150, 80 kDa
Positive control
purified XO-enzyme (Sigma)
Additional data
Additional Notes
We could only detect a 80kDa band in human liver cells (most likely the actual xanthine oxidase), whereas the positive control (purified enzyme) and kidney cell lysates showed the expected 150kDa band (xanthine oxidoreductase).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 17 2012