Anti-XRCC4抗体(ab97351)
Key features and details
- Rabbit polyclonal to XRCC4
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-XRCC4抗体
参阅全部 XRCC4 一抗 -
描述
兔多克隆抗体to XRCC4 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide, corresponding to a region within amino acids 266-279 of Human XRCC4 (NP_071801)
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阳性对照
- WB: HeLa cell nuclear extract. IHC-P: HBL435 xenograft tissue. IF: HeLa cell.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97351于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/1000.
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WB | (1) |
1/500 - 1/3000. Detects a band of approximately 55 kDa (predicted molecular weight: 38 kDa).
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ICC/IF | (2) |
1/100 - 1/1000.
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说明 |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Detects a band of approximately 55 kDa (predicted molecular weight: 38 kDa). |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the XRCC4 family. -
翻译后修饰
Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.
Monoubiquitinated.
Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7518 Human
- Omim: 194363 Human
- SwissProt: Q13426 Human
- Unigene: 567359 Human
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别名
- DNA double strand break repair and V(D)J recombination protein XRCC4 antibody
- DNA repair protein XRCC4 antibody
- SSMED antibody
see all
图片
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All lanes : Anti-XRCC4 antibody (ab97351) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa cell nuclear extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 38 kDa10% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded HBL435 xenograft using ab97351 at 1/500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling XRCC4 using ab97351 at 1/200 dilution. Lower image was also labelled with DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab97351 被引用在 5 文献中.
- Francica P et al. Functional Radiogenetic Profiling Implicates ERCC6L2 in Non-homologous End Joining. Cell Rep 32:108068 (2020). PubMed: 32846126
- Deraska PV et al. NF-?B inhibition by dimethylaminoparthenolide radiosensitizes non-small-cell lung carcinoma by blocking DNA double-strand break repair. Cell Death Discov 4:10 (2018). PubMed: 29531807
- Willis NA et al. Rad51 recruitment and exclusion of non-homologous end joining during homologous recombination at a Tus/Ter mammalian replication fork barrier. PLoS Genet 14:e1007486 (2018). PubMed: 30024881
- Stelcer E et al. Chondrocytes differentiated from human induced pluripotent stem cells: Response to ionizing radiation. PLoS One 13:e0205691 (2018). PubMed: 30352062
- Willis NA et al. Mechanism of tandem duplication formation in BRCA1-mutant cells. Nature 551:590-595 (2017). PubMed: 29168504