Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa cell)
Total protein in input
700 µg
Specification
HeLa cell
Immuno-precipitation step
Protein A/G
Other product details
Dilution
1/50
Incubation time
12 hour(s) and 0 minute(s)
Western Blot antibody
Western Blot antibody
None used
Additional data
Additional Notes
IP done on WCE HeLa with XPG ab46 clone 8h7 using 4 ug for 700 ug of total protein. IgG control antibody using 3 ug for 700ug of total protein. Imput is WCE Hela-50 ug
WB done with XPG antibody from Santa Cruz. ab46 in my experimental condition is not working in WB
WB done with XPG antibody from Santa Cruz. ab46 in my experimental condition is not working in WB
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Roberta Guglielmino
Verified customer
提交于 Apr 11 2007