Application
Western blot
Sample
Human Cell lysate - nuclear (fibroblast cell line)
Loading amount
100 µg
Specification
fibroblast cell line
Gel Running Conditions
Reduced Denaturing (6 %)
Blocking step
Blocking buffer from another company as blocking agent as blocking agent for 30 minute(s) · Concentration: 5µg/mL · Temperature: 21°C
Other product details
Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer from another company
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse
Host species: Goat
Clonality: Monoclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Monoclonal
Conjugation: Alkaline Phosphatase
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 87 kDa
Additional data
Additional Notes
Lanes:
1. GM00637 Cytosol
2. GM00637 Nucleus
3. Marker
1. GM00637 Cytosol
2. GM00637 Nucleus
3. Marker
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Annika Schäfer
Verified customer
提交于 Feb 10 2009