Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (RPE-1)
Specification
RPE-1
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100 in PBS
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200
Additional data
Additional Notes
Asynchronous but bleomycin treated RPE-1 cells were paraformaldehyde fixed (4%, 10min), permeablized and immunofluorescently labeled.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
提交于 Aug 16 2007