Anti-XBP1抗体(ab37152)
Key features and details
- Rabbit polyclonal to XBP1
- Suitable for: IHC-P, IHC-Fr, ICC, IHC (PFA fixed), WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-XBP1抗体
参阅全部 XBP1 一抗 -
描述
兔多克隆抗体to XBP1 -
宿主
Rabbit -
特异性
XBP-1 antibody will recognize both isoforms (non-spliced and spliced) of XBP-1. -
经测试应用
适用于: IHC-P, IHC-Fr, ICC, IHC (PFA fixed), WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow -
免疫原
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阳性对照
- WB: HepG2 cell lysate, A549 cell lysate. IHC-P: Human liver and non-lesional skin tissue; Mouse testis and spleen tissue, rat liver tissue. ICC/IF: HepG2 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab37152于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (12) |
Use a concentration of 5 µg/ml.
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IHC-Fr |
Use at an assay dependent concentration. PubMed: 20028872
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ICC |
Use a concentration of 10 µg/ml.
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IHC (PFA fixed) |
Use a concentration of 20 µg/ml.
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WB | (11) |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 29 kDa.Can be blocked with Human XBP1 peptide (ab39910).
Human samples only. Not batch tested in mouse or rat, please see AbReviews for more information. Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa (isoform 1) and 40 kD (isoform 2). Can be blocked with XBP1 peptide (ab39910). |
ICC/IF | (3) |
Use a concentration of 20 µg/ml.
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说明 |
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IHC-P
Use a concentration of 5 µg/ml. |
IHC-Fr
Use at an assay dependent concentration. PubMed: 20028872 |
ICC
Use a concentration of 10 µg/ml. |
IHC (PFA fixed)
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 29 kDa.Can be blocked with Human XBP1 peptide (ab39910). Human samples only. Not batch tested in mouse or rat, please see AbReviews for more information. Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa (isoform 1) and 40 kD (isoform 2). Can be blocked with XBP1 peptide (ab39910). |
ICC/IF
Use a concentration of 20 µg/ml. |
靶标
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功能
Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I. -
疾病相关
Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature. -
序列相似性
Belongs to the bZIP family.
Contains 1 bZIP domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7494 Human
- Entrez Gene: 22433 Mouse
- Entrez Gene: 289754 Rat
- Omim: 194355 Human
- SwissProt: P17861 Human
- SwissProt: O35426 Mouse
- SwissProt: Q9R1S4 Rat
- Unigene: 437638 Human
see all -
别名
- Tax responsive element binding protein 5 antibody
- Tax-responsive element-binding protein 5 antibody
- TREB5 antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded Rat Liver Tissue using ab37152 at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Lane 1 : Anti-XBP1 antibody (ab37152) at 0.25 µg/ml
Lane 2 : Anti-XBP1 antibody (ab37152) at 0.5 µg/ml
Lane 3 : Anti-XBP1 antibody (ab37152) at 1 µg/ml
All lanes : A549 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 29 kDa1h incubation at RT in 5% NFDM/TBST.
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Lane 1 : Anti-XBP1 antibody (ab37152) at 1 µg/ml
Lane 2 : Anti-XBP1 antibody (ab37152) at 2 µg/ml
All lanes : HepG2 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 29 kDa -
Immunohistochemistry of mouse testis cells labeling XBP1 with Anti-XBP1 antibody (ab37152) at 2μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4oC. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry of human liver tissue labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). DAPI staining (Blue).
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Immunohistochemistry of mouse spleen cells labeling XBP1 with Anti-XBP1 antibody (ab37152) at 2μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4oC. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry of mouse pancreas tissue labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). DAPI staining (Blue).
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Immunohistochemistry of human pancreas tissue labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). DAPI staining (Blue).
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Immunocytochemistry of HepG2 cells labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. Blue: DAPI staining.
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Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human liver tissue labeling XBP1 with Anti-XBP1 antibody (ab37152) at 5μg/ml.
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ab37152 staining XBP1 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate-EDTA antigen retrieval buffer and microwave. Heating was performed in the microwave 3 minutes on high until boiling, 6 minutes on 20% power. Samples were then blocked for 15 minutes at 27°C followed by incubation with the primary antibody at a 1/100 dilution for 12 hours at 4°C. An undiluted HRP-conjugated rat polyclonal was used as the secondary antibody.
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ab37152 at 1/200 dilution staining XBP1 in human non-lesional skin tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraformaldehyde tissue sections). A biotin labelled secondary was used at 1/100 dilution. Diaminobenzidine (DAB) was used for staining development in a ABC staining Kit and the sections were counterstained with haematoxylin.
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Immunocytochemistry of XBP-1 in HepG2 cells with ab37152 at 10 ug/mL
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (146)
ab37152 被引用在 146 文献中.
- Metzing UB et al. Endoplasmic reticulum stress and the unfolded protein response in skeletal muscle of subjects suffering from peritoneal sepsis. Sci Rep 12:504 (2022). PubMed: 35017615
- Shen J et al. Homocysteine facilitates endoplasmic reticulum stress and apoptosis of hepatocytes by suppressing ERO1α expression via cooperation between DNMT1 and G9a. Cell Biol Int 46:1236-1248 (2022). PubMed: 35347798
- Liu Z et al. XBP1 deficiency promotes hepatocyte pyroptosis by impairing mitophagy to activate mtDNA-cGAS-STING signaling in macrophages during acute liver injury. Redox Biol 52:102305 (2022). PubMed: 35367811
- Malik A et al. Interleukin-10 Mitigates Doxorubicin-Induced Endoplasmic Reticulum Stress as Well as Cardiomyopathy. Biomedicines 10:N/A (2022). PubMed: 35453640
- Zhang Y et al. Downregulated XBP-1 Rescues Cerebral Ischemia/Reperfusion Injury-Induced Pyroptosis via the NLRP3/Caspase-1/GSDMD Axis. Mediators Inflamm 2022:8007078 (2022). PubMed: 35497095