Application
Western blot
Sample
Rat Cell lysate - other (primary cortical neurons)
Loading amount
25 µg
Specification
primary cortical neurons
Blocking step
Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1x
Other product details
Dilution
1/1500
Incubation time
12 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: rockland IRdye800
Host species: Rabbit
Clonality: Polyclonal
Host species: Rabbit
Clonality: Polyclonal
Detection
Detection method
odyssey
Exposure
5 second(s)
Bands
Specific: 37, 50, 60 kDa
Positive control
samples: lane 1 untreated cytoplasmic extract, lane 2 untreated nuclear extract
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 09 2006