Anti-Wnt3a抗体[3A6] (ab81614)
Key features and details
- Mouse monoclonal [3A6] to Wnt3a
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Wnt3a抗体[3A6]
参阅全部 Wnt3a 一抗 -
描述
小鼠单克隆抗体[3A6] to Wnt3a -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length human Wnt3a (aa19-352) purified from E. coli (NP_149122).
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阳性对照
- WB: A549 cell lysates, Wnt3a transfected 293T cell lysate .
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常规说明
This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Ammonium Sulphate Precipitation -
克隆
单克隆 -
克隆编号
3A6 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81614于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
靶标
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功能
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. -
组织特异性
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. -
序列相似性
Belongs to the Wnt family. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 89780 Human
- Omim: 606359 Human
- SwissProt: P56704 Human
- Unigene: 336930 Human
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别名
- Protein Wnt 3a Precursor antibody
- Protein Wnt-3a antibody
- Wingless type MMTV integration site family member 3A antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab81614 被引用在 15 文献中.
- Wu CL et al. Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis. Nat Commun 12:362 (2021). PubMed: 33441552
- Tian X et al. Long noncoding RNA LINC00662 promotes M2 macrophage polarization and hepatocellular carcinoma progression via activating Wnt/ß-catenin signaling. Mol Oncol 14:462-483 (2020). PubMed: 31785055
- Ackers I et al. Oxidized Low-Density Lipoprotein Induces WNT5A Signaling Activation in THP-1 Derived Macrophages and a Human Aortic Vascular Smooth Muscle Cell Line. Front Cardiovasc Med 7:567837 (2020). PubMed: 33330641
- Jiang S et al. WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling. Cell Commun Signal 17:109 (2019). PubMed: 31462314
- Lyu X et al. microRNA-485 inhibits the malignant behaviors of retinoblastoma by directly targeting Wnt3a. Oncol Rep 41:3137-3147 (2019). PubMed: 30896857