Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Adult ovary and uterus)
Permeabilization
Yes - PBST (PBS / 0.1% v/v Triton X-100 (Sigma))
Specification
Adult ovary and uterus
Blocking step
3% donkey serum / 1% BSA (Life Technologies) in PBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
72 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% BSA / 3% donkey serum in PBST
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Donkey anti-Rabbit Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Image shows a section through the uterus of an adult mouse. Other tissues examined include sections through the ovary and through the posterior part of the E9.5 embryo. Unfortunately, this antibody did not work in the conditions/tissues we tried and further optimisation would be needed.
Abcam response
Thank you for sharing this data with us. ab96730 has previously been shown to work in IHC on paraffin embedded human lung sections (please see image on datasheet). However, based on this abreview, this antibody either does not work in IHC on frozen sections, or on mouse tissue, or both, under these experimental conditions. Abcam would welcome feedback from other researchers using ab96730 in their experiments.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 21 2015