Mouse, Rat, Rabbit, Cow
Recombinant fragment, corresponding to a region within amino acids 1-280 of Human Wnt11 (NP_004617).
293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji cell lysates for WB, HeLa cells for IF and Lung SCC for IHC-P.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
1/100 - 1/200.
1/100 - 1/250.
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Expressed in fetal lung, kidney, adult heart, liver, skeletal muscle, and pancreas.
Belongs to the Wnt family.
Palmitoylation at Ser-215 is required for efficient binding to frizzled receptors. Palmitoylation is necessary for proper trafficking to cell surface.
Secreted, extracellular space, extracellular matrix.
Information by UniProt
Protein Wnt-11 antibody
Wingless-related MMTV integration site 11 antibody
Western blot - Anti-Wnt11 antibody (ab96730)
Anti-Wnt11 antibody (ab96730) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 39 kDa 10% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (ab96730)
Immunohistochemical analysis of paraffin-embedded lung SCC, using ab96730 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (ab96730)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96730 at 1/200 dilution. Lower image merged with DNA probe.
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