antigen sequence corresponding to amino acids 257-363 of Human WSCD1.
- Human colon tissue; Transfected HEK293T cell WSCD1 over-expression lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
Belongs to the WSCD family.
Contains 2 WSC domains.
Information by UniProt
- WSC domain containing 1 antibody
- WSC domain-containing protein 1 antibody
- WSCD1 antibody
- WSCD1_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-WSCD1 antibody (ab121491)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121491 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WSCD1 antibody (ab121491)
ab121491, at 1/75 dilution, staining WSCD1 in paraffin-embedded Human colon tissue by Immunohistochemistry.
Western blot - Anti-WSCD1 antibody (ab121491)
All lanes : Anti-WSCD1 antibody (ab121491) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : WSCD1-transfected HEK293T over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique.
has not yet been referenced specifically in any publications.
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