Anti-WAPL/FOE抗体(ab70741)
Key features and details
- Rabbit polyclonal to WAPL/FOE
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-WAPL/FOE抗体
参阅全部 WAPL/FOE 一抗 -
描述
兔多克隆抗体to WAPL/FOE -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Rhesus monkey, Gorilla, Platypus -
免疫原
Synthetic peptide corresponding to Human WAPL/FOE aa 1125-1175.
Database link: Q7Z5K2 -
阳性对照
- HeLa nuclear extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70741于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/2500 - 1/10000. Detects a band of approximately 175 kDa (predicted molecular weight: 133 kDa).
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说明 |
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WB
1/2500 - 1/10000. Detects a band of approximately 175 kDa (predicted molecular weight: 133 kDa). |
靶标
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功能
Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. -
组织特异性
Isoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart. -
序列相似性
Contains 1 WAPL domain. -
细胞定位
Nucleus and Nucleus. Chromosome. Cytoplasm. Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase. - Information by UniProt
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数据库链接
- Entrez Gene: 23063 Human
- Entrez Gene: 218914 Mouse
- Entrez Gene: 290577 Rat
- Omim: 610754 Human
- SwissProt: Q7Z5K2 Human
- SwissProt: Q65Z40 Mouse
- Unigene: 203099 Human
- Unigene: 714876 Human
see all -
别名
- FOE antibody
- Friend of EBNA2 (Epstein Barr virus nuclear protein 2) antibody
- Friend of EBNA2 protein antibody
see all
图片
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All lanes : Anti-WAPL/FOE antibody (ab70741) at 0.2 µg/ml
Lane 1 : HeLa nuclear extract at 5 µg
Lane 2 : HeLa nuclear extract at 20 µg
Predicted band size: 133 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes -
All lanes : Anti-WAPL/FOE antibody (ab70741) at 1/2000 dilution
Lane 1 : Human U2OS cells
Lane 2 : Human U2OS cells treated with siRNA #1 against WAPL/FOE
Lane 3 : Human U2OS cells treated with siRNA #2 against WAPL/FOE
Lysates/proteins at 90 µg per lane.
Secondary
All lanes : Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 133 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab70741 被引用在 7 文献中.
- Shiimori M et al. Suv4-20h2 protects against influenza virus infection by suppression of chromatin loop formation. iScience 24:102660 (2021). PubMed: 34169237
- Kriz AJ et al. Balancing cohesin eviction and retention prevents aberrant chromosomal interactions, Polycomb-mediated repression, and X-inactivation. Mol Cell 81:1970-1987.e9 (2021). PubMed: 33725485
- Gu B et al. Opposing Effects of Cohesin and Transcription on CTCF Organization Revealed by Super-resolution Imaging. Mol Cell 80:699-711.e7 (2020). PubMed: 33091336
- Perea-Resa C et al. Cohesin Removal Reprograms Gene Expression upon Mitotic Entry. Mol Cell 78:127-140.e7 (2020). PubMed: 32035037
- Meisenberg C et al. Repression of Transcription at DNA Breaks Requires Cohesin throughout Interphase and Prevents Genome Instability. Mol Cell 73:212-223.e7 (2019). PubMed: 30554942
- Eykelenboom JK et al. Live imaging of marked chromosome regions reveals their dynamic resolution and compaction in mitosis. J Cell Biol 218:1531-1552 (2019). PubMed: 30858191
- Stelloh C et al. The cohesin-associated protein Wapal is required for proper Polycomb-mediated gene silencing. Epigenetics Chromatin 9:14 (2016). PubMed: 27087855