Anti-Vimentin抗体(ab39376)
Key features and details
- Chicken polyclonal to Vimentin
- Suitable for: ICC/IF
- Reacts with: Mouse
- Isotype: IgY
概述
-
产品名称
Anti-Vimentin抗体
参阅全部 Vimentin 一抗 -
描述
鸡多克隆抗体to Vimentin -
宿主
Chicken -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to a region of the Vimentin gene product shared between the mouse (NP_035831, NCBI) and human (NP_003371, NCBI) gene products.
-
阳性对照
- ICC/IF: Mouse brain
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: PBS
(10mM isotonic 0.9% w/v), pH 7.2 -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgY -
研究领域
相关产品
-
Isotype control
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab39376于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (5) |
1/100 - 1/200.
|
说明 |
---|
ICC/IF
1/100 - 1/200. |
靶标
-
功能
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. -
组织特异性
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. -
疾病相关
Cataract 30 -
序列相似性
Belongs to the intermediate filament family. -
结构域
The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. -
翻译后修饰
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 22352 Mouse
- SwissProt: P20152 Mouse
- Unigene: 268000 Mouse
-
形式
Vimentin is found in connective tissue and in the cytoskeleton. -
别名
- CTRCT30 antibody
- Epididymis luminal protein 113 antibody
- FLJ36605 antibody
see all
图片
数据表及文件
-
SDS download
-
Datasheet download
文献 (8)
ab39376 被引用在 8 文献中.
- Treviño-Villarreal JH et al. Down-Regulation of a Profibrotic Transforming Growth Factor-β1/Cellular Communication Network Factor 2/Matrix Metalloprotease 9 Axis by Triamcinolone Improves Idiopathic Subglottic Stenosis. Am J Pathol 191:1412-1430 (2021). PubMed: 34111429
- Sinkey RG et al. Thrombin-Induced Decidual Colony-Stimulating Factor-2 Promotes Abruption-Related Preterm Birth by Weakening Fetal Membranes. Am J Pathol 190:388-399 (2020). PubMed: 31955792
- Mong EF et al. Chromosome 19 microRNA cluster enhances cell reprogramming by inhibiting epithelial-to-mesenchymal transition. Sci Rep 10:3029 (2020). PubMed: 32080251
- Su N et al. Occurrence of Transmembrane Protein 119 in the Retina is Not Restricted to the Microglia: An Immunohistochemical Study. Transl Vis Sci Technol 8:29 (2019). PubMed: 31853425
- Guzeloglu-Kayisli O et al. Mechanisms of chorioamnionitis-associated preterm birth: interleukin-1β inhibits progesterone receptor expression in decidual cells. J Pathol 237:423-34 (2015). PubMed: 26175191
- Mulholland DJ et al. Pten loss and RAS/MAPK activation cooperate to promote EMT and metastasis initiated from prostate cancer stem/progenitor cells. Cancer Res 72:1878-89 (2012). IHC . PubMed: 22350410
- Schilte C et al. Type I IFN controls chikungunya virus via its action on nonhematopoietic cells. J Exp Med 207:429-42 (2010). IHC-Fr ; Mouse . PubMed: 20123960
- Lawson DA et al. Basal epithelial stem cells are efficient targets for prostate cancer initiation. Proc Natl Acad Sci U S A 107:2610-5 (2010). ICC/IF ; Mouse . PubMed: 20133806