Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Heart)
Specification
Heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/20
Incubation time
24 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 594 donkey anti?goat IgG
Host species: Donkey
Clonality: Polyclonal
Host species: Donkey
Clonality: Polyclonal
Additional data
Additional Notes
I have tried this Ab with overnight 4 deg primary Ab incubation or at 37 deg for 30min in both paraffin embedded and frozen dog heart tissue and it has been negative. To check that it was just this Ab I used another vimentin Ab (ab7783-500) against the same tissue sections and found that it worked well with either o/night or 37 deg incubation. In summary the goat p/clonal ab11256 does not work on paraffin-embedded formalin fixed(with heat mediated antigen retrieval) or frozen dog heart tissue.
Abcam response
Thank you for your interesting feedback. This product has not been tested for immunohistochemisry on formalin-fixed paraffin embedded sections and it seems that ab11256 may not be suitable for this type of application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 21 2006