Application
Western blot
Sample
Dog Cell lysate - whole cell (Osteosarcoma cell lines)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
50 µg
Specification
Osteosarcoma cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Dilution
1/500
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 53 kDa Non-specific: 25, 120 kDa
Positive control
143b- human osteosarcoma cell line
Additional data
Additional Notes
nitrocellulose membrane
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Holly Pondenis
Verified customer
提交于 Nov 21 2015