重组Anti-USO1抗体[EPR2140Y] (ab68137)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2140Y] to USO1
- Suitable for: WB
- Reacts with: Human, African green monkey
Related conjugates and formulations
概述
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产品名称
Anti-USO1抗体[EPR2140Y]
参阅全部 USO1 一抗 -
描述
兔单克隆抗体[EPR2140Y] to USO1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human, African green monkey
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human USO1 aa 1-100 (N terminal). The exact sequence is proprietary.
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阳性对照
- WB: HeLa cell lysate; 293T cell lysate; HepG2 cell lysate; Cos cell lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2140Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68137于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
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说明 |
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WB
1/1000 - 1/5000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa). |
靶标
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功能
General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity. -
序列相似性
Belongs to the VDP/USO1/EDE1 family.
Contains 10 ARM repeats. -
结构域
Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain. -
翻译后修饰
Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation. -
细胞定位
Cytoplasm > cytosol. Golgi apparatus membrane. Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes. - Information by UniProt
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数据库链接
- Entrez Gene: 8615 Human
- Entrez Gene: 56041 Mouse
- Entrez Gene: 56042 Rat
- Omim: 603344 Human
- SwissProt: O60763 Human
- SwissProt: Q9Z1Z0 Mouse
- SwissProt: P41542 Rat
- Unigene: 292689 Human
see all -
别名
- General vesicular transport factor antibody
- General vesicular transport factor p115 antibody
- P115 antibody
see all
图片
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All lanes : Anti-USO1 antibody [EPR2140Y] (ab68137) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Cos cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 108 kDa
Observed band size: 108 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab68137 被引用在 1 文献中.
- Jin Y & Dai Z USO1 promotes tumor progression via activating Erk pathway in multiple myeloma cells. Biomed Pharmacother 78:264-71 (2016). WB ; Human . PubMed: 26898451