Anti-AVPR1B抗体(ab104365)
Key features and details
- Rabbit polyclonal to AVPR1B
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-AVPR1B抗体
参阅全部 AVPR1B 一抗 -
描述
兔多克隆抗体to AVPR1B -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Mouse AVPR1B aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab124133) -
阳性对照
- This antibody gave a positive signal in NIH3T3 whole cell lysate as well as the following tissue lysates: E10 Mouse Embryo Brain; Mouse Forebrain; Human Brain Membrane
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab104365于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa). |
靶标
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功能
Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl-inositol-calcium second messenger system. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 553 Human
- Entrez Gene: 26361 Mouse
- Omim: 600264 Human
- SwissProt: P47901 Human
- SwissProt: Q9WU02 Mouse
- Unigene: 1372 Human
- Unigene: 89986 Mouse
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别名
- Antidiuretic hormone receptor 1b antibody
- Antidiuretic hormone, receptor for, V1B antibody
- Arginine vasopressin receptor 1B antibody
see all
图片
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All lanes : Anti-AVPR1B antibody (ab104365) at 1 µg/ml
Lane 1 : E10 Mouse Embryo Brain Tissue Lysate
Lane 2 : Forebrain (Mouse) Tissue Lysate
Lane 3 : Human brain normal tissue lysate - membrane extract (ab29456)
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 5 : E10 Mouse Embryo Brain Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 6 : Forebrain (Mouse) Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 7 : Human brain normal tissue lysate - membrane extract (ab29456) with Immunising peptide at 1 µg/ml
Lane 8 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 102 kDa, 22 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
VR1b contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab104365 被引用在 1 文献中.
- Dannenhoffer CA et al. Oxytocin and vasopressin modulation of social anxiety following adolescent intermittent ethanol exposure. Psychopharmacology (Berl) 235:3065-3077 (2018). PubMed: 30141056