Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cross section of mouse eye, looked at cornea)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS EDTA pH 9
Permeabilization
No
Specification
Cross section of mouse eye, looked at cornea
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
30 minute(s) · Temperature: 25°C · Diluent: 1.5% goat serum in PBS
Dilution
1/40
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti Rabbit biotinylated
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
This antibody worked great with the WR strain of the Vaccinia virus. I used an ABC kit for the blocking buffer, secondary antibody and A and B reagents. I used a Vector Labs Nova red kit for the stain and Gill's hematoxylin for the counter stain. Paraformaldehyde concentration was 4%
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Inna Larsen
Verified customer
提交于 Dec 29 2015