Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cell line)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
10 µg
Treatment
Transfected with pMD2.G or mock
Specification
HEK293 cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% BSA in PBS-T (0.1% Tween20)
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: goat anti-rabbit IgG Jackson Immunoresearch (#111-
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
HEK293 cells mock transfected "- VSVG"
Exposure
10 second(s)
Bands
Specific: 58 kDa
Positive control
HEK293 cells transfected with pMD2.G (VSVG encoding construct) "+ VSVG"
Additional data
Abcam response
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提交于 Sep 28 2015