Application
Flow Cytometry
Sample
Human Cell (Hela cells transfected with full length vsv-g)
Specification
Hela cells transfected with full length vsv-g
Preparation
Cell harvesting/tissue preparation method: Trypsin/centrifugation
Sample buffer: PBS 5%FCS
Sample buffer: PBS 5%FCS
Fixation
Formaldehyde
Permeabilization
Yes - 0.025% Triton-X in PBS
Gating Strategy
Size gated HeLa
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: 0.2% BSA, 0.1% sodium azide in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Streptavidin
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Hela cells transfected with full length vsv-g
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提交于 Sep 17 2010