Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skin, 3D skin model)
Permeabilization
Yes - 0.4% Triton X100
Specification
skin, 3D skin model
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Methanol
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% NGS, 3% BSA, 0.4% TritonX100 in PBS
Dilution
1/300
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
Epidermal keratinocyte is positive, as well as sensory neuron in the skin. Keratinocytes in a skin culture model is also positive.
PubMed ID
20060270,: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Sep 21 2011