Anti-TRPV1抗体(ab10296)
Key features and details
- Rabbit polyclonal to TRPV1
- Suitable for: IHC-FoFr
- Reacts with: Rat
- Isotype: IgG
概述
-
产品名称
Anti-TRPV1抗体
参阅全部 TRPV1 一抗 -
描述
兔多克隆抗体to TRPV1 -
宿主
Rabbit -
特异性
This antibody has been optimised on rat tissue. We recommend ab31895 for detection of TRPV1 in mouse tissue.
-
经测试应用
适用于: IHC-FoFrmore details -
种属反应性
与反应: Rat
预测可用于: Mouse, Non human primates -
免疫原
Synthetic peptide within Rat TRPV1 aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O35433 -
阳性对照
- IHC-Fr: rat dorsal root ganglion
-
常规说明
Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: Whole serum -
Concentration information loading...
-
纯度
Whole antiserum -
Primary antibody说明
Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10296于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-FoFr |
1/50 - 1/1000.
(see Guo et al reference). |
说明 |
---|
IHC-FoFr
1/50 - 1/1000. (see Guo et al reference). |
靶标
-
功能
Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis. -
组织特异性
Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level). -
序列相似性
Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
Contains 6 ANK repeats. -
结构域
The association domain (AD) is necessary for self-association. -
翻译后修饰
Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity. -
细胞定位
Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 193034 Mouse
- Entrez Gene: 83810 Rat
- SwissProt: Q704Y3 Mouse
- SwissProt: O35433 Rat
- Unigene: 447485 Mouse
- Unigene: 3073 Rat
-
别名
- Capsaicin receptor antibody
- DKFZp434K0220 antibody
- osm 9 like TRP channel 1 antibody
see all
图片
数据表及文件
-
SDS download
-
Datasheet download
文献 (8)
ab10296 被引用在 8 文献中.
- Serra MP et al. Anti-Inflammatory Effect of Beta-Caryophyllene Mediated by the Involvement of TRPV1, BDNF and trkB in the Rat Cerebral Cortex after Hypoperfusion/Reperfusion. Int J Mol Sci 23:N/A (2022). PubMed: 35408995
- Ju L et al. Corydalis Saxicola Bunting Total Alkaloids Attenuate Walker 256-Induced Bone Pain and Osteoclastogenesis by Suppressing RANKL-Induced NF-?B and c-Fos/NFATc1 Pathways in Rats. Front Pharmacol 11:609119 (2020). PubMed: 33574755
- Zhu TH et al. Mast cell stabilizer ketotifen reduces hyperalgesia in a rodent model of surgically induced endometriosis. J Pain Res 12:1359-1369 (2019). PubMed: 31118754
- Zhang J et al. Upregulation of TRPV1 in spinal dorsal root ganglion by activating NGF-TrkA pathway contributes to pelvic organ cross-sensitisation in rats with experimental autoimmune prostatitis. Andrologia 51:e13302 (2019). PubMed: 31074030
- Hulse RP et al. Regulation of alternative VEGF-A mRNA splicing is a therapeutic target for analgesia. Neurobiol Dis 71:245-59 (2014). IP ; Mouse . PubMed: 25151644
- van den Wijngaard RM et al. Essential role for TRPV1 in stress-induced (mast cell-dependent) colonic hypersensitivity in maternally separated rats. Neurogastroenterol Motil 21:1107-e94 (2009). IHC-FoFr ; Rat . PubMed: 19523146
- Chen W et al. Immortalization and characterization of a nociceptive dorsal root ganglion sensory neuronal line. J Peripher Nerv Syst 12:121-30 (2007). PubMed: 17565537
- Naeini RS et al. An N-terminal variant of Trpv1 channel is required for osmosensory transduction. Nat Neurosci 9:93-8 (2006). PubMed: 16327782