Mouse, Rat, Human
Synthetic peptide, corresponding to amino acids 1-221 of Human VPS28
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Detects a band of approximately 75 kDa (predicted molecular weight: 25 kDa).
Component of the ESCRT-I complex, a regulator of vesicular trafficking process.
Belongs to the VPS28 family.
Contains 1 VPS28 C-terminal domain. Contains 1 VPS28 N-terminal domain.
Cell membrane. Late endosome membrane.
Information by UniProt
ESCRT-I complex subunit VPS28 antibody
Vacuolar protein sorting 28 antibody
Western blot - Anti-VPS28 antibody (ab37729)
Anti-VPS28 antibody (ab37729) at 1/2000 dilution + VPS28 fusion protein at 0.5 µg
Goat anti-IgY-HRP at 1/1000 dilution
Predicted band size:
Observed band size:
75 kDa (
why is the actual band size different from the predicted?
Western blot analysis of E coli-derived fusion protein as test antigen. Western blot analysis of an E coli-derived fusion protein using ab37729. A colorimetric method was used for signal development. Observed band size may differ from predicted band size, since the test antigen is a fusion protein.
has not yet been referenced specifically in any publications.
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