Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (whole cell lysate)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
40 µg
Specification
whole cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk-TBS/T
Concentration
1 µg/ml
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Amersham ECL Mouse IgG, HRP-linked whole Ab (from
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ecl
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 95 kDa Non-specific: 50 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Sep 21 2017