Anti-VPAC2抗体(ab28624)
Key features and details
- Rabbit polyclonal to VPAC2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-VPAC2抗体
参阅全部 VPAC2 一抗 -
描述
兔多克隆抗体to VPAC2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide:
LQFHRGSRAQSFLQTETSVI
conjugated to KLH, corresponding to amino acids 419-438 of Human VIP Receptor 2 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28624于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/500.
|
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WB |
1/250 - 1/500. Predicted molecular weight: 49 kDa.
|
说明 |
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IHC-P
1/500. |
WB
1/250 - 1/500. Predicted molecular weight: 49 kDa. |
靶标
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功能
This is a receptor for VIP as well as PACAP-38 and -27, the activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Can be coupled to phospholipase C. -
组织特异性
Expressed in CD4+ T-cells, but not in CD8+ T-cells. Expressed in the T-cell lines Jurkat, PEER, MOLT-4, HSB, YT and Tsup-1, but not in the T-cell lines HARRIS and HUT 78. -
序列相似性
Belongs to the G-protein coupled receptor 2 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7434 Human
- Omim: 601970 Human
- SwissProt: P41587 Human
- Unigene: 585052 Human
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别名
- Helodermin-preferring VIP receptor antibody
- PACAP type III receptor antibody
- PACAP-R-3 antibody
see all
图片
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Anti-VPAC2 antibody (ab28624) at 1/500 dilution + PC-3 cell lysate at 30 µg
Secondary
Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/4000 dilution
Predicted band size: 49 kDa -
ab28624 staining Human VIP Receptor 2 in human neuroendocrine cells of the small intestine by immunohistochemistry using paraffin embedded tissue.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab28624 被引用在 9 文献中.
- Ivic I et al. VPAC1 receptors play a dominant role in PACAP-induced vasorelaxation in female mice. PLoS One 14:e0211433 (2019). PubMed: 30682157
- Jones JR et al. SCN VIP Neurons Are Essential for Normal Light-Mediated Resetting of the Circadian System. J Neurosci 38:7986-7995 (2018). PubMed: 30082421
- Liu D et al. mTOR signaling in VIP neurons regulates circadian clock synchrony and olfaction. Proc Natl Acad Sci U S A 115:E3296-E3304 (2018). PubMed: 29555746
- Bertolini M et al. Vasoactive intestinal peptide, whose receptor-mediated signalling may be defective in alopecia areata, provides protection from hair follicle immune privilege collapse. Br J Dermatol 175:531-41 (2016). PubMed: 27059672
- Doi M et al. Gpr176 is a Gz-linked orphan G-protein-coupled receptor that sets the pace of circadian behaviour. Nat Commun 7:10583 (2016). PubMed: 26882873
- Juhász T et al. Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures. Int J Mol Sci 16:17344-67 (2015). PubMed: 26230691
- Miller JE et al. Vasoactive intestinal polypeptide mediates circadian rhythms in mammalian olfactory bulb and olfaction. J Neurosci 34:6040-6 (2014). PubMed: 24760863
- An S et al. Spatiotemporal distribution of vasoactive intestinal polypeptide receptor 2 in mouse suprachiasmatic nucleus. J Comp Neurol 520:2730-41 (2012). PubMed: 22684939
- Ortner A et al. VPAC receptor mediated tumor cell targeting by protamine based nanoparticles. J Drug Target 18:457-67 (2010). ICC ; Human . PubMed: 20050817