Anti-VMA2抗体[13D11B2] (ab113684)
Key features and details
- Mouse monoclonal [13D11B2] to VMA2
- Suitable for: ICC/IF, WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
概述
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产品名称
Anti-VMA2抗体[13D11B2] -
描述
小鼠单克隆抗体[13D11B2] to VMA2 -
宿主
Mouse -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Saccharomyces cerevisiae -
免疫原
Full length native protein (purified). This information is considered to be commercially sensitive.
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阳性对照
- Yeast membranes
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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纯度
Proprietary Purification -
纯化说明
Near homogeneity as judged by SDS-PAGE (purity >95%). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
克隆
单克隆 -
克隆编号
13D11B2 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113684于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
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说明 |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
靶标
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功能
Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. It is an electrogenic proton pump that generates a proton motive force of 180 mv, inside positive and acidic, in the vacuolar membrane vesicles. -
序列相似性
Belongs to the ATPase alpha/beta chains family. - Information by UniProt
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数据库链接
- Entrez Gene: 852424 Saccharomyces cerevisiae
- SwissProt: P16140 Saccharomyces cerevisiae
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别名
- V-ATPase 57 kDa subunit antibody
- V-ATPase subunit B antibody
- V-type proton ATPase subunit B antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab113684 被引用在 2 文献中.
- Calvo IA et al. The fission yeast FLCN/FNIP complex augments TORC1 repression or activation in response to amino acid (AA) availability. iScience 24:103338 (2021). PubMed: 34805795
- Belgareh-Touzé N et al. Ubiquitination of ERMES components by the E3 ligase Rsp5 is involved in mitophagy. Autophagy 13:114-132 (2017). WB ; Saccharomyces cerevisiae . PubMed: 27846375