Application
Western blot
Sample
Human Purified protein (culture medium from astrocytes)
Loading amount
10 µg
Specification
culture medium from astrocytes
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse HRP conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 27 kDa
Additional data
Additional Notes
10 ml culture medium from fibroblasts and astrocytes was concentrated 100 folds by filter centrifugation, 10 ul aliquot was loaded on gel to determine the secreted VEGF.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 23 2010