Anti-VEGF Receptor 2抗体(ab11939)
Key features and details
- Rabbit polyclonal to VEGF Receptor 2
- Suitable for: Sandwich ELISA, IP, ELISA, Neutralising, WB, Flow Cyt, IHC-Fr
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-VEGF Receptor 2抗体
参阅全部 VEGF Receptor 2 一抗 -
描述
兔多克隆抗体to VEGF Receptor 2 -
宿主
Rabbit -
特异性
The polyclonal antibody will detect native and recombinant human VEGFR2/KDR in ELISA experiments and on the surface or solubilized from different human cell types. -
经测试应用
适用于: Sandwich ELISA, IP, ELISA, Neutralising, WB, Flow Cyt, IHC-Frmore details -
种属反应性
与反应: Mouse, Rat, Human, Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human VEGF Receptor 2. Highly pure recombinant human soluble extracellular domain of KDR (110 kDa) (D1-D7).
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阳性对照
- IHC: Human spleen Flow Cyt: HUVEC (Human umbilical vein endothelial cell line) whole cell lysate and HDLEC (Human dermal lymphatic endothelial cell line) whole cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
This antibody is affinity purified from rabbit sera. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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sELISA pair antibody
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11939于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 (ab42228).
For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab42228 as Capture. |
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IP |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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Neutralising |
Use at an assay dependent concentration. PubMed: 17991870
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WB | (1) |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 151 kDa.
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Flow Cyt |
Use a concentration of 1 - 5 µg/ml.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-Fr |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 (ab42228). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab42228 as Capture. |
IP
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
Neutralising
Use at an assay dependent concentration. PubMed: 17991870 |
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 151 kDa. |
Flow Cyt
Use a concentration of 1 - 5 µg/ml. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-Fr
Use at an assay dependent concentration. |
靶标
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功能
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
疾病相关
Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3791 Human
- Entrez Gene: 16542 Mouse
- Entrez Gene: 25589 Rat
- Omim: 191306 Human
- SwissProt: P35968 Human
- SwissProt: P35918 Mouse
- SwissProt: O08775 Rat
- Unigene: 479756 Human
see all -
别名
- CD309 antibody
- CD309 antigen antibody
- EC 2.7.10.1 antibody
see all
图片
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Immunohistochemistry (Frozen sections) analysis of human spleen tissue sections labelling VEGF Receptor 2 with ab11939.
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All lanes : Anti-VEGF Receptor 2 antibody (ab11939)
Lane 1 : recombinant human soluble VEGFR-2
Lane 2 : recombinant native VEGFR-2
Secondary
All lanes : anti-rabbit AP-conjugated antibody
Predicted band size: 151 kDaSamples were loaded in 10% SDS-PAGE under reducing conditions.
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Flow Cytometry analysis of primary human dermal lymphatic endothelial cells (HDLEC) labelling VEGF Receptor 2 with ab11939.
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Flow Cytometry analysis of primary human umbilical vein endothelial cells (HUVEC) labelling VEGF Receptor 2 with ab11939.
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Immunohistochemistry (Frozen sections) analysis of human spleen tissue sections labelling VEGF Receptor 2 with ab11939.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (53)
ab11939 被引用在 53 文献中.
- Lan J et al. Cord Blood Natural Killer Cells Inhibit Sepsis Caused by Feces-Induced Acute Peritonitis via Increasing Endothelium Integrity. Cell Transplant 31:9636897221090257 (2022). PubMed: 35438589
- Abedpoor N et al. Cross Brain-Gut Analysis Highlighted Hub Genes and LncRNA Networks Differentially Modified During Leucine Consumption and Endurance Exercise in Mice with Depression-Like Behaviors. Mol Neurobiol 59:4106-4123 (2022). PubMed: 35476290
- Li P et al. Extracellular vesicle-encapsulated microRNA-424 exerts inhibitory function in ovarian cancer by targeting MYB. J Transl Med 19:4 (2021). PubMed: 33407591
- He S et al. Multiple targeted self-emulsifying compound RGO reveals obvious anti-tumor potential in hepatocellular carcinoma. Mol Ther Oncolytics 22:604-616 (2021). PubMed: 34589579
- Zhong N et al. Apatinib inhibits the growth of small cell lung cancer via a mechanism mediated by VEGF, PI3K/Akt and Ki-67/CD31. J Cell Mol Med 25:10039-10048 (2021). PubMed: 34590406