重组Anti-VEGF Receptor 1抗体[Y103] (ab32152)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y103] to VEGF Receptor 1
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human, Chinese hamster
Related conjugates and formulations
概述
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产品名称
Anti-VEGF Receptor 1抗体[Y103]
参阅全部 VEGF Receptor 1 一抗 -
描述
兔单克隆抗体[Y103] to VEGF Receptor 1 -
宿主
Rabbit -
特异性
Based on the antibody's immunogen sequence, it recognises 151 kDa VEGF receptor 1/Flt1, splice isoforms sFlt1 (77 kDa) and sFlt1-14 (82 kDa), and isoform 4 (61 kDa). The sequence is not present in isoforms 5-8 based on Uniprot ID P17948. -
经测试应用
适用于: WB, IHC-P, IPmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Mouse, Rat, Human, Chinese hamster -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human and mouse brain tissue; IHC-P: Human gastric carcinoma tissue; IHC-Fr: Mouse brain tissue; IP: Mouse brain lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Y103 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32152于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (10) |
1/1000 - 1/5000. Predicted molecular weight: 151 kDa.
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IHC-P | (6) |
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
1/30 - 1/100.
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说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 151 kDa. |
IHC-P
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
1/30 - 1/100. |
靶标
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功能
Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis. -
组织特异性
Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform sFlt1 is strongly expressed in placenta. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2321 Human
- Entrez Gene: 14254 Mouse
- Entrez Gene: 54251 Rat
- Omim: 165070 Human
- SwissProt: P17948 Human
- SwissProt: P35969 Mouse
- SwissProt: P53767 Rat
- Unigene: 594454 Human
see all -
别名
- EC 2.7.10.1 antibody
- FLT 1 antibody
- FLT antibody
see all
图片
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All lanes : Anti-VEGF Receptor 1 antibody [Y103] (ab32152) at 1/1000 dilution
Lane 1 : Mouse brain lysates
Lane 2 : Human brain lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 151 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 40 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling VEGF with ab32152, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human gastric carcinoma. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
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VEGF Receptor 1 was immunoprecipitated from 0.35 mg mouse brain lysate 10μg with ab32152 at 1:30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab32152 1:1000 dilution (2 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
Lane 1: Mouse brain lysate 10μg.
Lane 2: ab32152 IP in mouse brain lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab32152 in mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human abdominal aortic aneurysm (AAA) wall tissue sections labeling VEGF Receptor 1 with ab32152 at 1/100 dilution.
Resected aortic tissues were immersed in 10% neutral buffered formalin for at least 24 h for immunohistochemical staining. Tissue sample was embedded in paraffin; 4 µm sections were cut and mounted onto MAS-coated slides. The sections were deparaffinized, dehydrated, and boiled in a pressure cooker in 0.01 M citric acid buffer (pH 6.0) for 20 min. The sections were washed with phosphate-buffered saline and incubated with 3% H2O2 in absolute methanol for 5 min to inhibit any endogenous peroxidase activity. Sections were preincubated with 3% normal goat serum for 20 min to minimize nonspecific binding to VEGF Receptor 1, and incubated with ab32152 at 4°C overnight in a moist chamber. The section was washed with phosphate-buffered saline and then incubated with the appropriate secondary antibody for 30 min at room temperature. Staining was visualized with Vector DAB, and tissue section was then counterstained with hematoxylin.
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Anti-VEGF Receptor 1 antibody [Y103] (ab32152) at 1/10000 dilution + mouse brain tissue
Predicted band size: 151 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (276)
ab32152 被引用在 276 文献中.
- Chen C et al. miRNA-576-5p promotes endometrial cancer cell growth and metastasis by targeting ZBTB4. Clin Transl Oncol 25:706-720 (2023). PubMed: 36538280
- Sun YL et al. Therapeutic effects of menstrual blood-derived endometrial stem cells on mouse models of streptozotocin-induced type 1 diabetes. World J Stem Cells 14:104-116 (2022). PubMed: 35126831
- Chen JW et al. Identification of circRNAs involved in the development of hepatocellular carcinoma after insufficient radiofrequency ablation. Neoplasma 69:527-537 (2022). PubMed: 35144476
- Sari AN et al. A Low Dose Combination of Withaferin A and Caffeic Acid Phenethyl Ester Possesses Anti-Metastatic Potential In Vitro: Molecular Targets and Mechanisms. Cancers (Basel) 14:N/A (2022). PubMed: 35159054
- Yen JH et al. Improved Wound Healing by Naringin Associated with MMP and the VEGF Pathway. Molecules 27:N/A (2022). PubMed: 35268795