Application
Western blot
Sample
Mouse Tissue lysate - whole (lung)
Gel Running Conditions
Reduced Denaturing (6%)
Loading amount
30 µg
Specification
lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% non fat milk - 1XTBS-tween
Dilution
1/500
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: anti rabbit-HRP
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
luminata
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: none kDa Non-specific: 60, 70, 80 kDa
Positive control
stripped and used vinculin-HRP antibody and bands were very strong in all samples
Additional data
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested for cross reactivity with mouse and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Sep 09 2016