Application
Immunohistochemistry (Frozen sections)
Sample
Sheep Tissue sections (Lung-Pulmonary Artery)
Permeabilization
Yes - Triton-100
Specification
Lung-Pulmonary Artery
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Acetone
Other product details
Incubation time
4 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: 1X PBS, Donkey serum 5%, BSA 3%
Concentration
5 µg/ml
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: AP182F | Donkey Anti-Rabbit IgG Antibody, FITC con
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Additional data
Additional Notes
Antibody is too non-specific in sheep tissue application. Staining is not localized, and in areas other than that we would consider to be actual VE-Cadherin. Non-conclusive WB information.
Abcam response
We would like to thank this researcher for submitting their Abreview. The antibody had not yet been tested with sheep and we were unsure if there would be cross reactivity with this species. We appreciate the time and effort that the reviewer has put in to testing this product. They were provided with a discount code under our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Christina Valued Customer
Verified customer
提交于 Nov 21 2015