Anti-VCP抗体(ab111740)
Key features and details
- Rabbit polyclonal to VCP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-VCP抗体
参阅全部 VCP 一抗 -
描述
兔多克隆抗体to VCP -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish -
免疫原
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 290 and 507 of human VCP (P55072)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab111740于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
1/500 - 1/20000. Predicted molecular weight: 89 kDa.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/20000. Predicted molecular weight: 89 kDa. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Regulates E3 ubiquitin-protein ligase activity of RNF19A. -
疾病相关
Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) [MIM:167320]; also known as muscular dystrophy, limb-girdle, with Paget disease of bone or pagetoid amyotrophic lateral sclerosis or pagetoid neuroskeletal syndrome or lower motor neuron degeneration with Paget-like bone disease. IBMPFD features adult-onset proximal and distal muscle weakness (clinically resembling limb girdle muscular dystrophy), early-onset Paget disease of bone in most cases and premature frontotemporal dementia. -
序列相似性
Belongs to the AAA ATPase family. -
翻译后修饰
Phosphorylated by tyrosine kinases in response to T-cell antigen receptor activation (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated. -
细胞定位
Cytoplasm > cytosol. Nucleus. Present in the neuronal hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients. - Information by UniProt
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数据库链接
- Entrez Gene: 507345 Cow
- Entrez Gene: 7415 Human
- Entrez Gene: 269523 Mouse
- Entrez Gene: 397524 Pig
- Entrez Gene: 116643 Rat
- Entrez Gene: 380491 Xenopus laevis
- Entrez Gene: 327197 Zebrafish
- Omim: 601023 Human
see all -
别名
- 15S Mg(2+) ATPase p97 subunit antibody
- 15S Mg(2+)-ATPase p97 subunit antibody
- ALS14 antibody
see all
图片
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All lanes : Anti-VCP antibody (ab111740) at 1/1000 dilution
Lane 1 : NT2D1 whole cell lysate
Lane 2 : U87-MG whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 89 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of VCP in paraformaldehyde fixed MCF7, using ab111740 at a 1/500 dilution. Lower image shows cells costained with Hoechst 33342.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab111740 被引用在 1 文献中.
- Jongkamonwiwat N et al. Noise Exposures Causing Hearing Loss Generate Proteotoxic Stress and Activate the Proteostasis Network. Cell Rep 33:108431 (2020). PubMed: 33238128