APC Anti-VCAM1抗体[STA],prediluted (ab103173)
Key features and details
- APC Mouse monoclonal [STA] to VCAM1, prediluted
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
概述
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产品名称
APC Anti-VCAM1抗体[STA],prediluted
参阅全部 VCAM1 一抗 -
描述
APC小鼠单克隆抗体[STA] to VCAM1,prediluted -
宿主
Mouse -
偶联物
APC. Ex: 645nm, Em: 660nm -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human VCAM1. Human DS6 T cell line
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阳性对照
- Flow Cyt: TNF-alpha activated HUVEC cells; Treated A549 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading...
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纯度
Size exclusion -
纯化说明
Conjugates are made from highly purified antibodies (>95% by SDS-PAGE). -
克隆
单克隆 -
克隆编号
STA -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab103173于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use 10µl for 106 cells.
or 100µl whole blood
ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
说明 |
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Flow Cyt
Use 10µl for 106 cells. or 100µl whole blood
ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
靶标
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功能
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. -
组织特异性
Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. -
序列相似性
Contains 7 Ig-like C2-type (immunoglobulin-like) domains. -
结构域
Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion. -
翻译后修饰
Sialoglycoprotein. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7412 Human
- GenBank: NP_001069.1 Human
- GenBank: NP_001186763.1 Human
- Omim: 192225 Human
- SwissProt: P19320 Human
- Unigene: 109225 Human
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别名
- CD106 antibody
- CD106 Antigen antibody
- INCAM 100 antibody
see all
图片
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Flow cytometry overlay histogram showing wild-type A549 (green line) and VCAM1 knockout A549 cells (red line, ab273758), treated with 10 ng/ml TNF-alpha (ab259410) for 16 h (left) and untreated (right), stained with ab103173. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab103173) (1x106 in 100μl at 0.2μg/ml) for 30 min at 4°C.
Isotype control antibody mouse IgG1κ Allophycocyanin was used at the same concentration and conditions as the primary antibody (wild-type A549 - black line; VCAM knockout A549 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.
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Surface staining of HDLM-2 cells with ab103173.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab103173 被引用在 2 文献中.
- Yuan X et al. SDF‑1α/CXCR4 signaling promotes capillary tube formation of human retinal vascular endothelial cells by activating ERK1/2 and PI3K pathways in vitro. Mol Med Rep 26:N/A (2022). PubMed: 35946444
- Leca G et al. Expression of VCAM-1 (CD106) by a subset of TCR gamma delta-bearing lymphocyte clones. Involvement of a metalloprotease in the specific hydrolytic release of the soluble isoform. J Immunol 154:1069-77 (1995). PubMed: 7529789