重组PE Anti-VCAM1抗体[EPR5047] (ab223981)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR5047] to VCAM1
- Suitable for: Flow Cyt (Intra)
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
概述
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产品名称
PE Anti-VCAM1抗体[EPR5047]
参阅全部 VCAM1 一抗 -
描述
PE兔单克隆抗体[EPR5047] to VCAM1 -
宿主
Rabbit -
偶联物
PE. Ex: 488nm, Em: 575nm -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Mouse
预测可用于: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt (intra): NIH3T3 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5047 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-VCAM1 antibody [EPR5047] (ab134047)
- Alexa Fluor® 647 Anti-VCAM1 antibody [EPR5047] (ab194319)
- HRP Anti-VCAM1 antibody [EPR5047] (ab195540)
- Anti-VCAM1 antibody [EPR5047] - Low endotoxin, Azide free (ab215380)
- FITC Anti-VCAM1 antibody [EPR5047] (ab223982)
- APC Anti-VCAM1 antibody [EPR5047] (ab223983)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223981于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/1000.
The cellular localisation of this product has been verified in ICC/IF. |
说明 |
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Flow Cyt (Intra)
1/1000. The cellular localisation of this product has been verified in ICC/IF. |
靶标
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功能
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. -
组织特异性
Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. -
序列相似性
Contains 7 Ig-like C2-type (immunoglobulin-like) domains. -
结构域
Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion. -
翻译后修饰
Sialoglycoprotein. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7412 Human
- Entrez Gene: 22329 Mouse
- Entrez Gene: 25361 Rat
- GenBank: NP_001069.1 Human
- GenBank: NP_001186763.1 Human
- Omim: 192225 Human
- SwissProt: P19320 Human
- SwissProt: P29533 Mouse
see all -
别名
- CD106 antibody
- CD106 Antigen antibody
- INCAM 100 antibody
see all
图片
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Overlay histogram showing NIH3T3 cells stained with ab223981 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223981, 1/1000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab223981 被引用在 1 文献中.
- Zhao Y et al. Mesenchymal Stem Cells Ameliorate Fibrosis by Enhancing Autophagy via Inhibiting Galectin-3/Akt/mTOR Pathway and by Alleviating the EMT via Inhibiting Galectin-3/Akt/GSK3β/Snail Pathway in NRK-52E Fibrosis. Int J Stem Cells 16:52-65 (2023). PubMed: 35483714