Application
Western blot
Sample
Human Cell lysate - whole cell (human and mouse cell lines)
Loading amount
50 µg
Specification
human and mouse cell lines
Gel Running Conditions
Reduced Denaturing (8)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST + 1:1000 sodium azide
Secondary antibody
Name
Non-Abcam antibody was used: GE antirabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/7500
Detection
Detection method
Millipore HRP substrate
Exposure
30 second(s)
Bands
Specific: 97 kDa
Positive control
Jurkat
Additional data
Additional Notes
Lane 1 H16N2 whole cell lysate
Lane 2 H16N2 whole cell lysate
Lane 3 H16N2 whole cell lysate
Lane 4 H16N2 whole cell lysate
Lane 5 HEK293T whole cell lysate
Lane 6 HEK293T whole cell lysate
Lane 7 HEK293T whole cell lysate
Lane 8 HEK293T whole cell lysate
Lane 9 HEK293T whole cell lysate
Lane10 HEK293T whole cell lysate
Lane 11 Jurkat Cytosolic cell lysate
Lane 2 H16N2 whole cell lysate
Lane 3 H16N2 whole cell lysate
Lane 4 H16N2 whole cell lysate
Lane 5 HEK293T whole cell lysate
Lane 6 HEK293T whole cell lysate
Lane 7 HEK293T whole cell lysate
Lane 8 HEK293T whole cell lysate
Lane 9 HEK293T whole cell lysate
Lane10 HEK293T whole cell lysate
Lane 11 Jurkat Cytosolic cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 28 2010