重组Anti-VAV1 (phospho Y174)抗体[EP510Y] (ab76225)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP510Y] to VAV1 (phospho Y174)
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, Dot blot
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-VAV1 (phospho Y174)抗体[EP510Y]
参阅全部 VAV1 一抗 -
描述
兔单克隆抗体[EP510Y] to VAV1 (phospho Y174) -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IF, Dot blotmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human VAV1 (phospho Y174). The exact sequence is proprietary.
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阳性对照
- WB: Jurkat cell lysate treated with pervanadate. ICC/IF: Jurkat cell lysate treated with pervanadate. Flow Cyt (intra): WB: Jurkat cell lysate treated with pervanadate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP510Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76225于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB | (1) |
1/1000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
|
ICC/IF |
1/100 - 1/500.
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Dot blot |
Use at an assay dependent concentration.
|
说明 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa). |
ICC/IF
1/100 - 1/500. |
Dot blot
Use at an assay dependent concentration. |
靶标
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功能
Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation. -
组织特异性
Widely expressed in hematopoietic cells but not in other cell types. -
序列相似性
Contains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
结构域
The DH domain is involved in interaction with CCPG1. -
翻译后修饰
Phosphorylated on tyrosine residues. - Information by UniProt
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数据库链接
- Entrez Gene: 7409 Human
- Omim: 164875 Human
- SwissProt: P15498 Human
- Unigene: 116237 Human
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别名
- Oncogene vav antibody
- p95Vav antibody
- Proto-oncogene vav antibody
see all
图片
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All lanes : Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : Jurkat treated with 50mM pervanadate for 5minutes whole cell lysate
Lane 3 : Jurkat treated with 50mM pervanadate for 5min whole cell lysate 10 µg. Then the membrane was incubated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 101 kDaBlocking and diluting buffer: 5% NFDM/TBST
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Immunocytochemistry/Immunofluorescence analysis of Untreated Jurkat cells and Pervanadate (1mM,30min) treated Jurkat cells labelling VAV1 (phospho Y174) with purified ab76225 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100, and counterstained with ab7291 anti-Tubulin (mouse mAb) 1:1000 (1ug/ml). An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Negative Control 1: Rabbit primary antibody and anti-mouse secondary antibody(ab150120)
Negative Control 2: Mouse primary antibody(ab7291) and anti-rabbit secondary antibody(ab150077)
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Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) untreated/treated with 1mM pervanadate for 30min cells labeling VAV1 with unpurified ab76225 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. Untreated control (Green).
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Dot blot analysis of VAV1 (pY174) peptide (Lane 1) and VAV1 non-phospho peptide (Lane 2) labelling VAV1 (phospho Y174) with ab76225 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab76225 被引用在 17 文献中.
- Cortes JR et al. Oncogenic Vav1-Myo1f induces therapeutically targetable macrophage-rich tumor microenvironment in peripheral T cell lymphoma. Cell Rep 39:110695 (2022). PubMed: 35443168
- Raiter A et al. A novel role for an old target: CD45 for breast cancer immunotherapy. Oncoimmunology 10:1929725 (2021). PubMed: 34104545
- Alon D et al. Downregulation of CD45 Signaling in COVID-19 Patients Is Reversed by C24D, a Novel CD45 Targeting Peptide. Front Med (Lausanne) 8:675963 (2021). PubMed: 34414199
- Guedan S et al. Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability. J Clin Invest 130:3087-3097 (2020). PubMed: 32069268
- Lyu J et al. Thymic-specific regulation of TCR signaling by Tespa1. Cell Mol Immunol 16:897-907 (2019). PubMed: 31316154