Anti-VAV1抗体(ab97574)
Key features and details
- Rabbit polyclonal to VAV1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-VAV1抗体
参阅全部 VAV1 一抗 -
描述
兔多克隆抗体to VAV1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 83 and 538 of Human VAV1 (NP_005419)
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阳性对照
- WB: Jurkat, NCI-H929 and BCL-1 whole cell lysate. IHC-P: Human serous ovarian cancer.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97574于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 98 kDa.
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IHC-P |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 98 kDa. |
IHC-P
1/100 - 1/1000. |
靶标
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功能
Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation. -
组织特异性
Widely expressed in hematopoietic cells but not in other cell types. -
序列相似性
Contains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
结构域
The DH domain is involved in interaction with CCPG1. -
翻译后修饰
Phosphorylated on tyrosine residues. - Information by UniProt
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数据库链接
- Entrez Gene: 7409 Human
- Entrez Gene: 22324 Mouse
- Omim: 164875 Human
- SwissProt: P15498 Human
- SwissProt: P27870 Mouse
- Unigene: 116237 Human
- Unigene: 248172 Mouse
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别名
- Oncogene vav antibody
- p95Vav antibody
- Proto-oncogene vav antibody
see all
图片
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All lanes : Anti-VAV1 antibody (ab97574) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 98 kDa7.5% SDS-PAGE
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Paraffin-embedded human serous ovarian cancer tissue stained VAV1 using ab97574 at 1/100 dilution in immunohistochemical analysis.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min
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Anti-VAV1 antibody (ab97574) at 1/1000 dilution + BCL-1 (mouse B lymphocyte lymphoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 98 kDa7.5% SDS-PAGE
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All lanes : Anti-VAV1 antibody (ab97574) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 98 kDa7.5% SDS-PAGE
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab97574 被引用在 7 文献中.
- Zhu X et al. TMT-Based Quantitative Proteomic Analysis Reveals Downregulation of ITGAL and Syk by the Effects of Cycloastragenol in OVA-Induced Asthmatic Mice. Oxid Med Cell Longev 2022:6842530 (2022). PubMed: 36329800
- He B et al. MicroRNA-326 decreases tau phosphorylation and neuron apoptosis through inhibition of the JNK signaling pathway by targeting VAV1 in Alzheimer's disease. J Cell Physiol 235:480-493 (2020). PubMed: 31385301
- Liu Y et al. Stearic acid methyl ester ?promotes migration of mesenchymal stem cells and accelerates cartilage defect repair. J Orthop Translat 22:81-91 (2020). PubMed: 32440503
- Li Y et al. Human iPSC-Derived Natural Killer Cells Engineered with Chimeric Antigen Receptors Enhance Anti-tumor Activity. Cell Stem Cell 23:181-192.e5 (2018). PubMed: 30082067
- Zhou Y et al. Protective effects of microRNA-330 on amyloid β-protein production, oxidative stress, and mitochondrial dysfunction in Alzheimer's disease by targeting VAV1 via the MAPK signaling pathway. J Cell Biochem 119:5437-5448 (2018). PubMed: 29369410
- Kang L et al. Elevated level of Vav1 was correlated with advanced biological behavior and poor prognosis in patients with gastric cancer. Int J Clin Exp Pathol 11:391-398 (2018). PubMed: 31938123
- Boddicker RL et al. Integrated mate-pair and RNA sequencing identifies novel, targetable gene fusions in peripheral T-cell lymphoma. Blood 128:1234-45 (2016). PubMed: 27297792