Anti-VASP (phospho S157)抗体[5C6] (ab58555)
Key features and details
- Mouse monoclonal [5C6] to VASP (phospho S157)
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-VASP (phospho S157)抗体[5C6]
参阅全部 VASP 一抗 -
描述
小鼠单克隆抗体[5C6] to VASP (phospho S157) -
宿主
Mouse -
特异性
This antibody recognizes the the ~50 kDa VASP protein phosphorylated at Ser157. It does not cross-react with non-phosphorylated VASP or with un-related phosphorylation sites. -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human VASP (phospho S157) conjugated to keyhole limpet haemocyanin.
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阳性对照
- Vanadate-treated A431 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide -
Concentration information loading...
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纯度
Size exclusion -
纯化说明
Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. -
克隆
单克隆 -
克隆编号
5C6 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58555于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use 0.5µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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Flow Cyt
Use 0.5µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. -
组织特异性
Highly expressed in platelets. -
序列相似性
Belongs to the Ena/VASP family.
Contains 1 WH1 domain. -
结构域
The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
The WH1 domain mediates interaction with XIRP1. -
翻译后修饰
Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1. Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239. Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation. Carbon monoxide (CO) promotes phosphorylation at Ser-157, while nitric oxide (NO) promotes phosphorylation at Ser-157, but also at Ser-239. Response to NO and CO is blunted in platelets from diabetic patients, and VASP is not phosphorylated efficiently at Ser-157 and Ser-239. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions. - Information by UniProt
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数据库链接
- Entrez Gene: 7408 Human
- Omim: 601703 Human
- SwissProt: P50552 Human
- Unigene: 515469 Human
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别名
- Vasodilator stimulated phosphoprotein antibody
- Vasodilator-stimulated phosphoprotein antibody
- VASP antibody
- VASP_HUMAN antibody
图片
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Overlay histogram showing THP1 cells stained with ab58555 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58555, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab58555 被引用在 7 文献中.
- O'Banion CP et al. Compartmentalized cAMP Generation by Engineered Photoactivated Adenylyl Cyclases. Cell Chem Biol 26:1393-1406.e7 (2019). PubMed: 31353320
- O'Banion CP et al. Design, construction, and validation of optogenetic proteins. Methods Enzymol 621:171-190 (2019). PubMed: 31128778
- O'Banion CP et al. Design and Profiling of a Subcellular Targeted Optogenetic cAMP-Dependent Protein Kinase. Cell Chem Biol 25:100-109.e8 (2018). PubMed: 29104065
- Li XF et al. Downregulation of the long noncoding RNA MBNL1-AS1 protects sevoflurane-pretreated mice against ischemia-reperfusion injury by targeting KCNMA1. Exp Mol Med 50:115 (2018). PubMed: 30185781
- Neukamm SS et al. Phosphorylation of serine 1137/1138 of mouse insulin receptor substrate (IRS) 2 regulates cAMP-dependent binding to 14-3-3 proteins and IRS2 protein degradation. J Biol Chem 288:16403-15 (2013). Human . PubMed: 23615913
- Mise N et al. Zyxin is a transforming growth factor-ß (TGF-ß)/Smad3 target gene that regulates lung cancer cell motility via integrin a5ß1. J Biol Chem 287:31393-405 (2012). WB . PubMed: 22778267
- Martin-Padura I et al. Spontaneous cell fusion of acute leukemia cells and macrophages observed in cells with leukemic potential. Neoplasia 14:1057-66 (2012). PubMed: 23226099