重组PE Anti-VAMP8/EDB抗体[EP2629Y] (ab208831)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EP2629Y] to VAMP8/EDB
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
概述
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产品名称
PE Anti-VAMP8/EDB抗体[EP2629Y]
参阅全部 VAMP8/EDB 一抗 -
描述
PE兔单克隆抗体[EP2629Y] to VAMP8/EDB -
宿主
Rabbit -
偶联物
PE. Ex: 488nm, Em: 575nm -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt (intra): HeLa cells.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP2629Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab208831于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/2500.
The cellular localisation of this product has been verified in ICC/IF |
说明 |
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Flow Cyt (Intra)
1/2500. The cellular localisation of this product has been verified in ICC/IF |
靶标
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功能
Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes. -
组织特异性
Platelets. -
序列相似性
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 8673 Human
- Entrez Gene: 22320 Mouse
- Entrez Gene: 83730 Rat
- Omim: 603177 Human
- SwissProt: Q9BV40 Human
- SwissProt: O70404 Mouse
- SwissProt: Q9WUF4 Rat
- Unigene: 714302 Human
see all -
别名
- EDB antibody
- Endobrevin antibody
- VAMP 8 antibody
see all
图片
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Overlay histogram showing HeLa cells stained with ab208831 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208831, 1/2500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab208831 尚未被引用在任何文献中。