Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cortex cell line)
Loading amount
30 µg
Specification
Cortex cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: Vector
Host species: Horse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 120 kD kDa Non-specific: 30 kDa
Positive control
Cell line expressing V5 tagged Protein (120kD)
Negative control
Cell line (untreated)
Additional data
Additional Notes
can be used at higher dilution than 1:1000
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 02 2010